Membrane filter method for recovery of fecal coliforms in chlorinated sewage effluents
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چکیده
منابع مشابه
Membrane filter method for recovery of fecal coliforms in chlorinated sewage effluents.
The standard one-step M-FC broth-membrane-filter procedure for recovery of fecal coliforms from chlorinated sewage effluents is much less effective than the multiple-tube (most-probable-number) technique. A two-step membrane-filter method, using a pre-enrichment technique with phenol red lactose broth and incubation at 35 degrees C for 4 h, followether 18+/-2 h, enhanced fecal coliform recovery...
متن کاملEvaluation of Methods for Detecting Coliforms and Fecal Streptococci in Chlorinated Sewage Effluents
Total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) recoveries in chlorinated secondary sewage effluents were investigated by the membrane filter (MF) and multiple-tube (most probable number, MPN) methods. The LES two-step MF method was found to be comparable to the MPN procedure for determining TC. The TC detection was 1.5 times greater by the LES two-step technique than tha...
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Fecal coliforms growing in a selective lactose-based broth medium at 44.5 degrees C generate a change in the electrical impedance of the culture relative to a sterile control when populations reach 10(6) to 10(7) per ml. The ratio of these changes was measured automatically, and the data were processed by computer. A linear relation was found between the log10 of the number of fecal coliforms i...
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A single-step most-probable-number method for enumerating fecal coliforms in sewage treatment plant effluents is described. The method requires the use of only one lactose-based medium and a single incubation temperature of 44.5 degrees C, and it can be completed in 18 h or less, as compared with up to 72 h for the standard most-probable-number method. The appearance of growth is the sole crite...
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A two-layer membrane filtration (MF) medium (injury-mitigating MF [IM-MF]) and a procedure for the enumeration of injured fecal coliforms are described. These procedures included the addition of glycerol and acetate plus reducing agents to both layers of a two-layer medium and rinsing of the filter with a rich resuscitation medium. Some changes in incubation time and temperatures were used. Thi...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1976
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.32.4.547-552.1976